Expression of NOS and VEGF in feline mammary tumours and their correlation with angiogenesis     

Islam MS  Matsumoto M  Hidaka R  Miyoshi N  Yasuda N 《Veterinary journal (London, England : 1997)》2012,192(3):338-344

In order to define the role of nitric oxide (NO) in feline mammary tumours, the expression of endothelial or inducible nitric oxide synthase (e/iNOS) and vascular endothelial growth factor (VEGF), and their relationship with angiogenesis, was investigated in 23 feline mammary tumours (two hyperplastic, 19 adenocarcinoma, one osteosarcoma and one squamous cell carcinoma) by immunohistochemistry. Tumour angiogenesis was assessed by CD31 immunostaining and was expressed as microvessel density (MVD). In general, iNOS immunoreactivity was localised in tumour cells and occasionally in stromal myofibroblasts, whereas eNOS and VEGF were localised in the cytoplasm of tumour epithelial cells and endothelium. In malignancy, expression of iNOS increased from well- to less-differentiated phenotypes (Grades 1-3) and was significantly higher in G3 and G2 when compared with G1 cases. However, increasing eNOS expression was limited only in hyperplastic lesions and showed no significant changes among the grades. In addition, expression of iNOS was positively correlated with VEGF and MVD in feline mammary tumours and both measures were significantly greater in less differentiated phenotypes (P<0.05). In conclusion, the expression of NOS isoforms in feline mammary tumours depended on tumour grade, and the positive correlations between iNOS and angiogenic markers suggests that iNOS synthesised by tumour cells promotes tumour growth. Thus, iNOS can be used as an important immunohistochemical marker to determine the degree of malignancy and prognosis of feline mammary carcinoma.  相似文献   

Effects of single-tree selection harvesting on hymenopteran and saproxylic insect assemblages in the canopy and understory of northern temperate forests     

Sandy M. Smith  Nurul Islam  M. Isabel Bellocq 《林业研究》2012,23(2):275-284

Insects respond to changes in microhabitat caused by canopy disturbance, and thus can be used to examine the ecological impacts of harvesting. Single-tree selection harvesting is the most common silvicultural system used to emulate local small-scale natural disturbance and maintain uneven-aged forest structure in temperate forests. Here, we test for differences in richness, abundance, and composition of hymenopteran and saproxylic insect assemblages at four different taxon levels (selected insect orders; and all hymenopteran families, and braconid subfamilies and morphospecies) between the canopy and understory of unharvested and single-tree selection harvested sites in a northern temperate forest from central Canada. Harvesting had no effect on insect assemblage richness, composition or abundance at the three highest taxon levels (order, family and subfamily). Similarly, richness and abundance at the lowest-taxon level (braconid morphospecies) were similar, although composition differed slightly between unharvested and harvested stands. Insect assemblages were vertically stratified, with generally higher abundance (for Diptera, Hymenoptera, some hymenopteran families and braconid subfamilies) and richness (for braconid morphospecies) in the understory than the canopy. In particular, composition of the braconid morphospecies assemblage showed relatively low similarity between the understory and canopy. Single-tree selection harvesting appears to influ-ence wood-associated insect taxa only subtly through small changes in community composition at the lowest taxon level, and thus is recommended as a conservative approach for managing these northern temperate forests.  相似文献   

Molecular cloning and gene transcription analyses of barwin‐type PR‐4 genes from Phellinus sulphurascens‐infected Douglas‐fir seedlings     

M. A. Islam  R. N. Sturrock  A. K. M. Ekramoddoullah 《Forest Pathology》2012,42(4):279-288

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Development and testing of a surface roughness measurement device based on aerial ultrasonic reflections     

Okajima  Kenji  Nagaoka  Seiya  Islam  Mohammad Raihanul  Ito  Ryouei  Watanabe  Ken 《Paddy and Water Environment》2020,18(2):345-353

Paddy and Water Environment - Repairs of concrete irrigation channels in Japan are guided to a large extent by the degree to which their walls have degraded over decades of use. Current methods of...  相似文献   

Soil organic carbon storage and quality are impacted by corn cob biochar application on a tropical sandy loam     

Amoakwah  Emmanuel  Arthur  Emmanuel  Frimpong  Kwame A.  Parikh  Sanjai J.  Islam  Rafiq 《Journal of Soils and Sediments》2020,20(4):1960-1969

Purpose

Humic substances, which are integral components of total organic carbon (TOC), influence soil quality. The study aimed to investigate whether humic and non-humic fractions exhibit early, consistent, and measurable changes and affect TOC sensitivity and storage in a tropical sandy loam soils amended with corn cob biochar.

Materials and methods

There were four treatments with four replicates established in a randomized complete block design. Composite soil samples were taken from plots without biochar (CT), from plots incorporated with 15 t biochar ha^?1 (BC-15), and 30 t biochar ha^?1 without or with phosphate fertilizer (BC-30 and BC-30+P). The TOC, and humin, humic acid (HA), and fulvic acid (HA) fractions of soil organic carbon were determined for each treatment. The optical densities (400–700 nm) were measured on the soil-free extracts by spectrophotometry; the densities measured at 465 and 665 nm were used to calculate the E₄₆₅/E₆₆₅ ratios.

Results and discussion

The BC-30 and BC-30+P plots recorded the highest TOC, humin, humic acid (HA), and fulvic acid (FA) contents with respect to the lowest in the CT. The total exchangeable carbon stratification was significantly higher in all the biochar-treated plots relative to the CT. Spectral analysis showed higher values of E₄₆₅/E₆₆₅ (5.02 and 5.15) in the CT and BC-15-treated soils, respectively, compared with the BC-30 and BC-30+P-amended soils with E₄₆₅/E₆₆₅ ratios of 2.76 and 2.98, respectively.

Conclusions

Corn cob biochar applied to a tropical sandy loam:

? increased the concentrations of HA and FA and led to increased stratification of TOC, with a stronger effect on HA compared with FA;

? significantly lowered E₄₆₅/E₆₆₅ at the high biochar application rate of 30 t ha^?1, implying the dominance of high molecular weight humic acid-like substances, and increased degree of aromaticity of the TOC.

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Genetic diversity of Bangladeshi native chickens based on complete sequence of mitochondrial DNA D-loop region     

M. A. Islam  S. A. M. Osman  M. Nishibori 《British poultry science》2019,60(6):628-637

ABSTRACT

1. The aim of this study was to explore genetic diversity and possible origin of Bangladeshi (BD) native chickens. The complete mtDNA D-loop region was sequenced in 60 chickens representing five populations; naked neck, full feathered, Aseel, Hilly and autosomal dwarf. The 61 reference sequences representing different domestic chicken clades in China, India, Laos, Indonesia, Myanmar, and other Eurasian regions were included. The mtDNA D-loop sequence polymorphism and maternal origin of five BD populations were analysed.

2. A total of 35 polymorphic sites, and 21 haplotypes were detected in 60 mtDNA D-loop sequences. The haplotype and nucleotide diversity of the five populations were 0.921 ± 0.018 and 0.0061 ± 0.0019, respectively. Both mtDNA network and phylogenetic analysis indicated four clades (four haplogroups) in BD populations (21 haplotypes) along with 61 reference haplotypes. Clade E contained the most individuals (20) and haplotypes (11) of BD chickens, followed by clade D (17, 6), clade C (12, 2) and clade F (11, 2), respectively.

3. The higher number of unique haplotypes found in Yunnan, China, suggested that the origin of BD chickens was in this region. The haplotypes from different haplogroups were introduced in Bangladeshi chickens from India, China and Myanmar. The phylogenetic tree showed a close relationship of BD chickens with the clusters from India, China, Myanmar and Laos, and indicated the dispersion of BD chickens from these sources. The phylogenetic information revealed high genetic diversity of BD chickens because of their origin from different lineages with high genetic variation and distance, which was determined from four cluster and neighbour-joining trees.

4. In conclusion, BD populations had high genetic diversity. The mtDNA network profiles and phylogenetic trees showed multiple maternal origins of BD chickens from India, China, Myanmar and Laos.  相似文献   

Effect of potassium fertilization on yield and potassium nutrition of Boro rice in a wetland ecosystem of Bangladesh     

Aminul Islam  Abdul Muttaleb 《Archives of Agronomy and Soil Science》2016,62(11):1530-1540

Effect of potassium (K) fertilization (0, 20, 40, 60, 80 and 100 kg K ha^?1) on yield, nitrogen (N) and K nutrition of Boro (dry season) rice and apparent soil K balance was studied. Experiment was conducted at Bangladesh Rice Research Institute (BRRI) regional station farm, Habiganj, Bangladesh during 2007–2008 to 2009–2010 in a wetland rice ecosystem under haor area. Cropping pattern was Boro–Fallow–Fallow. A popular rice variety BRRI dhan29 was tested in a randomized complete block design with three replications. Results indicated that BRRI dhan29 maintained an average grain yield of 5.19 t ha^?1 year^?1 without K fertilization. Potassium fertilization significantly increased the grain yield to 6.86 t ha^?1 year^?1. Quadratic equations best explained the progressive increase of rice yield with increasing K rates. Optimum dose of K in 3 years ranged from 78 to 93 kg ha^?1. Internal N use efficiency of rice decreased with increasing K rates. However, K use efficiency was inconsistent. Apparent K balance study revealed that application of 100 kg K ha^?1 was not able to maintain a positive K balance in soil under wetland ecosystem with Boro–Fallow–Fallow cropping system. However, K fertilization decreased the negativity of K balance in soil.  相似文献   

Plant strengtheners enhance parasitoid attraction to herbivore‐damaged cotton via qualitative and quantitative changes in induced volatiles          下载免费PDF全文

Islam S Sobhy  Matthias Erb  Ted CJ Turlings 《Pest management science》2015,71(5):686-693

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Leptosphaeria spp., phoma stem canker and potential spread of L. maculans on oilseed rape crops in China     

X. Zhang  R. P. White  E. Demir  M. Jedryczka  R. M. Lange  M. Islam  Z. Q. Li  Y. J. Huang  A. M. Hall  G. Zhou  Z. Wang  X. Cai  P. Skelsey  B. D. L. Fitt 《Plant pathology》2014,63(3):598-612

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Efficacy of strain RB51 vaccine in protecting infection and vertical transmission against Brucella abortus in Sprague-Dawley rats     

Md. Ariful Islam  Mst. Minara Khatun  Byeong-Kirl Baek  Sung-Il Lee 《Journal of veterinary science (Suw?n-si, Korea)》2009,10(3):211-218

Immunizing animals in the wild against Brucella (B.) abortus is essential to control bovine brucellosis because cattle can get the disease through close contact with infected wildlife. The aim of this experiment was to evaluate the effectiveness of the B. abortus strain RB51 vaccine in protecting infection as well as vertical transmission in Sprague-Dawley (SD) rats against B. abortus biotype 1. Virgin female SD rats (n = 48) two months of age were divided into two groups: one group (n = 24) received RB51 vaccine intraperitoneally with 3 × 10¹⁰ colony forming units (CFU) and the other group (n = 24) was used as non-vaccinated control. Non-vaccinated and RB51-vaccinated rats were challenged with 1.5 × 10⁹ CFU of virulent B. abortus biotype 1 six weeks after vaccination. Three weeks after challenge, all rats were bred. Verification of RB51-vaccine induced protection in SD rats was determined by bacteriological, serological and molecular screening of maternal and fetal tissues at necropsy. The RB51 vaccine elicited 81.25% protection in SD rats against infection with B. abortus biotype 1. Offspring from rats vaccinated with RB51 had a decreased (p < 0.05) prevalence of vertical transmission of B. abortus biotype 1 compared to the offspring from non-vaccinated rats (20.23% and 87.50%, respectively). This is the first report of RB51 vaccination efficacy against the vertical transmission of B. abortus in the SD rat model.  相似文献